A spokeswoman said: "We are currently dealing with an ongoing incident at Glasgow Airport involving a 737 aircraft following reports of smoke within the cabin.
"Emergency services are in attendance and the passengers have been evacuated as a precaution."
A statement from Jet2 said: "This morning's flight LS177 from Glasgow Airport to Alicante made an emergency stop on the runway just prior to take-off.
"The aircraft was forced to curtail take-off due to smoke in the cabin.
"All 189 passengers have disembarked the aircraft."