"She sounded okay. How did she not tell me she needed help? I'll never get over this. She didn't sound as if... you know. If she needed help, you would say 'Help me'. She said 'Get my dad'. She wouldn't speak to me because I was screaming."
Her daughter told them she would phone again later that evening but the call never came.
Ms Spence has not been seen since April 2011 and prosecutors allege Colin Coats, 42, David Parker, 38, Philip Wade, 42, and Paul Smith, 47, abducted, tortured and murdered her. All four deny the charges against them.
At their trial at the High Court in Glasgow, Mrs Spence also told jurors that Coats made her and her husband hand over their caravan in Burntisland, Fife, because he believed Ms Spence owed him money.
Coats, Parker, Wade and Smith are accused of taking Ms Spence from a street in Glasgow on April 14 2011. They are said to have held her hostage at a flat in West Kilbride, Ayrshire, where it is alleged they bound, gagged and assaulted her in an apparent attempt to extract financial information from her.