Clydesdale Bank has been named the Scottish Network Group of the Year and Lothian and Borders Fire and Rescue Service is awarded Most Improved Employer of the Year. Royal Bank of Scotland also made the Top 100, Stonewall said.
The full results will be announced at a ceremony in Edinburgh tonight, hosted by the arts funding body Creative Scotland.
Colin Macfarlane, director of Stonewall Scotland, said: "Every employer in the Top 100 has performed impressively.
"These results are significant as it's just 10 years since we won vital legal protections for gay people in the workplace.
"By seeking to recruit, develop and retain the very best talent regardless of sexual orientation, the Scottish employers featured in the Top 100 are helping to make our workplaces fair and equal in our lifetime."